Phytoncides (Wood Essential Oils) Induce Human Natural Killer Cell Activity

  title={Phytoncides (Wood Essential Oils) Induce Human Natural Killer Cell Activity},
  author={Qing Li and Ari Nakadai and Hiroki Matsushima and Yoshifumi Miyazaki and Alan M. Krensky and Tomoyuki Kawada and Kanehisa Morimoto},
  journal={Immunopharmacology and Immunotoxicology},
  pages={319 - 333}
To explore the effect of forest bathing on the human immune system, we investigated the effect of phytoncides (wood essential oils) on natural killer (NK) activity and the expression of perforin, granzyme A and granulysin in human NK cells. [] Key Method We used NK-92MI cell, an interleukin-2 independent human NK cell line derived from the NK-92 cell, in the present study. NK-92MI cells express the CD56 surface marker, perforin, granzyme A, and granulysin by flow cytometry and are highly cytotoxic to K562…
Effect of Phytoncide from Trees on Human Natural Killer Cell Function
Findings indicate that phytoncide exposure and decreased stress hormone levels may partially contribute to increased NK activity.
Effect of ziram on natural killer, lymphokine-activated killer, and cytotoxic T lymphocyte activity
Findings suggest the ziram-induced inhibition of NK, LAK, and CTL activities to be at least partially mediated by decreases in the intracellular levels of Gr3/K, granulysin, perforin, GrA, and GrB.
α-Pinene Enhances the Anticancer Activity of Natural Killer Cells via ERK/AKT Pathway
It is demonstrated that α-pinene activates NK cells and increases NK cell cytotoxicity, suggesting it is a potential compound for cancer immunotherapy.
Effect of kumquat (Fortunella crassifolia) pericarp on natural killer cell activity in vitro and in vivo
Results indicate that raw kumquat pericarp acetone fraction activates NK cells in vitro and in vivo, suggesting that NK cell activation effects of KP-AF may be caused by carotenoids such as β-cryptoxanthin.
Natural Killer (NK) Cell Assays in Immunotoxicity Testing.
  • Qing Li
  • Biology
    Methods in molecular biology
  • 2018
This chapter will describe the methods for NK cell assays in immunotoxicity testing and the intracellular levels of perforin, granzymes, and granulysin determined by flow cytometry used in the evaluation of NK cell function.
Soluble components of Hericium erinaceum induce NK cell activation via production of interleukin-12 in mice splenocytes
WEHE indirectly activates the cytolytic ability of NK cells via the induction of IL-12 in total splenocytes, and possibly via other immuno-mediators or cellular components.
Effect of carbamate pesticides on perforin, granzymes A-B-3/K, and granulysin in human natural killer cells
It was found that all carbamate pesticides significantly reduced the intracellular levels of perforin, GrA, GrB, Gr3/K, and GRN in NK-92CI cells in a dose-dependent manner, however, the strength of the effect differed among the pesticides, and the order was thiram < ziram > maneb > carbaryl.
The mechanism of organophosphorus pesticide-induced inhibition of cytolytic activity of killer cells.
This study reviews the new mechanism of OP-induced inhibition of activities of natural killer (NK), lymphokine-activated killer (LAK) and cytotoxic T lymphocytes (CTL) and reports on how OPs induce apoptosis of immune cells.


Granulysin in human serum as a marker of cell‐mediated immunity
The results suggest that granulysin is well associated with diverse activities of NK cells and CTL in physiological and pathological settings and could be a useful novel serum marker to evaluate the overall status of host cellular immunity.
Characterization of genetically altered, interleukin 2-independent natural killer cell lines suitable for adoptive cellular immunotherapy.
Data presented here show that by particle-mediated gene transfer two IL-2-independent variants of NK-92 are created that are identical to parental cells in virtually all respects, including high cytotoxic activity, which should allow prolonged treatment with fully active natural killer cells without the need for exogenous IL- 2 support.
Cytolytic T-cell cytotoxicity is mediated through perforin and Fas lytic pathways
Two complementary, specific cytotoxic mechanisms are functional in CTLs, one based on the secretion of lytic proteins and one which depends on cell-surface ligand–receptor interaction, which is triggered upon T-cell receptor occupancy and directed to the cognate target cell.
Characterization of a human cell line (NK-92) with phenotypical and functional characteristics of activated natural killer cells.
Growth of the cell line (termed NK-92) is dependent on the presence of recombinant IL-2 and a dose as low as 10 U/ml is sufficient to maintain proliferation; cells die within 72 h when deprived of IL- 2; IL-7 and IL-12 do not maintain long-term growth, although IL- 7 induces short-term proliferation measured by 3H-thymidine incorporation.
Tumor Immunity in Perforin-Deficient Mice: A Role for CD95 (Fas/APO-1)1
The finding of up-regulated CD95 expression on tumor cells placed in vivo suggests that a CD95-based mechanism plays a role in tumor immunity at early stages of tumor growth, and suggests that the progressive down-regulation ofCD95 expression during tumor progression may indeed be an escape mechanism as previously reported.
Intracellular Mediators of Granulysin-Induced Cell Death 1
It is shown that Ca2+ and K+ channels as well as reactive oxygen species are involved in granulysin-mediated Jurkat cell death, and an increase in intracellular glutathione protects target cells from granuly sin-induced lysis, indicating the importance of the redox state in granules-mediated cell death.
An antimicrobial activity of cytolytic T cells mediated by granulysin.
The ability of CTLs to kill intracellular M. tuberculosis was dependent on the presence of granulysin in cytotoxic granules, defining a mechanism by which T cells directly contribute to immunity against intrACEllular pathogens.