Phytodetoxification of TNT by transgenic plants expressing a bacterial nitroreductase

@article{Hannink2001PhytodetoxificationOT,
  title={Phytodetoxification of TNT by transgenic plants expressing a bacterial nitroreductase},
  author={Nerissa K. Hannink and Susan J. Rosser and Christopher E. French and Amrik Basran and James A. H. Murray and Stephen Nicklin and Neil C. Bruce},
  journal={Nature Biotechnology},
  year={2001},
  volume={19},
  pages={1168-1172}
}
There is major international concern over the wide-scale contamination of soil and associated ground water by persistent explosives residues. [...] Key Result Transgenic plants expressing nitroreductase show a striking increase in ability to tolerate, take up, and detoxify TNT. Our work suggests that expression of nitroreductase (NR) in plants suitable for phytoremediation could facilitate the effective cleanup of sites contaminated with high levels of explosives.Expand
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Transgenic tobacco was created constitutively expressing the nsfI nitroreductase gene from Enterobacter cloacae and its high levels appeared to correlate with enhanced tolerance and transformation of TNT, indicating enhanced detoxification compared to the wild type. Expand
Phytoremediation of Toxic Explosives
Widespread contamination of the environment by explosives resulting from the manufacture, disposal and testing of munitions is becoming a matter of increasing concern. Most explosives are consideredExpand
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A system was designed to overcome high phytotoxicity of TNT by expressing a NAD(P)H-flavin nitroreductase from Enterobacter cloacae to investigate the possibility of TNT phytoremediation, and the resulting transgenic Arabidopsis showed a remarkable improvement in the ability to tolerate, absorb, and detoxify TNT. Expand
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NfsI transplastomic tobacco (Nicotiana tabacum L.) is developed to reduce pollen-borne transgene flow and is shown to be significantly more tolerant to TNT, produced increased biomass and removed more TNT from the media than untransformed plants. Expand
Degradation of trinitrotoluene by transgenic nitroreductase in Arabidopsis plants
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This is the first study to demonstrate the use of transgenic plants to tackle two chemically diverse organic compounds at levels comparable with those found on contaminated training ranges, indicating that this technology is capable of remediating concentrations of RDX found in’situ. Expand
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TLDR
Transgenic aspen expressing pnrA are highly interesting for phytoremediation applications on contaminated soil and underground aquifers. Expand
Enhancing phytoremediation through the use of transgenics and endophytes.
  • S. Doty
  • Biology, Medicine
  • The New phytologist
  • 2008
TLDR
This review examines the recent advances in enhancing phytoremediation through transgenic plant research and through the use of symbiotic endophytic microorganisms within plant tissues. Expand
Biodegradation of explosives by transgenic plants
Abstract Environmental contamination by explosives like TNT, RDX, and HMX, due to their manufacturing, testing, and disposal, is becoming a matter of immense interest, worldwide. Most explosives areExpand
Transgenic plants for enhanced phytoremediation of toxic explosives.
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  • Medicine
  • Current opinion in biotechnology
  • 2009
TLDR
Transgenic plants exhibiting biodegradation capabilities of microorganisms bring the promise of an efficient and environmental-friendly technology for cleaning up polluted soils. Expand
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