Phytodetoxification of TNT by transgenic plants expressing a bacterial nitroreductase

  title={Phytodetoxification of TNT by transgenic plants expressing a bacterial nitroreductase},
  author={Nerissa K. Hannink and Susan J. Rosser and Christopher E. French and Amrik Basran and James A. H. Murray and Stephen Nicklin and Neil C. Bruce},
  journal={Nature Biotechnology},
There is major international concern over the wide-scale contamination of soil and associated ground water by persistent explosives residues. [...] Key Result Transgenic plants expressing nitroreductase show a striking increase in ability to tolerate, take up, and detoxify TNT. Our work suggests that expression of nitroreductase (NR) in plants suitable for phytoremediation could facilitate the effective cleanup of sites contaminated with high levels of explosives.Expand
Enhanced Transformation of TNT by Tobacco Plants Expressing a Bacterial Nitroreductase
Transgenic tobacco was created constitutively expressing the nsfI nitroreductase gene from Enterobacter cloacae and its high levels appeared to correlate with enhanced tolerance and transformation of TNT, indicating enhanced detoxification compared to the wild type. Expand
Phytoremediation of Toxic Explosives
Widespread contamination of the environment by explosives resulting from the manufacture, disposal and testing of munitions is becoming a matter of increasing concern. Most explosives are consideredExpand
Phytoremediation of 2,4,6-trinitrotoluene by Arabidopsis plants expressing a NAD(P)H-flavin nitroreductase from Enterobacter cloacae
A system was designed to overcome high phytotoxicity of TNT by expressing a NAD(P)H-flavin nitroreductase from Enterobacter cloacae to investigate the possibility of TNT phytoremediation, and the resulting transgenic Arabidopsis showed a remarkable improvement in the ability to tolerate, absorb, and detoxify TNT. Expand
Phytodetoxification of TNT by transplastomic tobacco (Nicotiana tabacum) expressing a bacterial nitroreductase
NfsI transplastomic tobacco (Nicotiana tabacum L.) is developed to reduce pollen-borne transgene flow and is shown to be significantly more tolerant to TNT, produced increased biomass and removed more TNT from the media than untransformed plants. Expand
Degradation of trinitrotoluene by transgenic nitroreductase in Arabidopsis plants
Zhu B., Han H.J., Fu X.Y., Li Z.J., Gao J.J., Yao Q.H. (2018): Degradation of trinitrotoluene by transgenic nitroreductase in Arabidopsis plants. Plant Soil Environ., 64: 379–385. The explosiveExpand
Engineering plants for the phytoremediation of RDX in the presence of the co-contaminating explosive TNT.
This is the first study to demonstrate the use of transgenic plants to tackle two chemically diverse organic compounds at levels comparable with those found on contaminated training ranges, indicating that this technology is capable of remediating concentrations of RDX found in’situ. Expand
Bioremediation of 2,4,6-trinitrotoluene by bacterial nitroreductase expressing transgenic aspen.
Transgenic aspen expressing pnrA are highly interesting for phytoremediation applications on contaminated soil and underground aquifers. Expand
Enhancing phytoremediation through the use of transgenics and endophytes.
  • S. Doty
  • Biology, Medicine
  • The New phytologist
  • 2008
This review examines the recent advances in enhancing phytoremediation through transgenic plant research and through the use of symbiotic endophytic microorganisms within plant tissues. Expand
Biodegradation of explosives by transgenic plants
Abstract Environmental contamination by explosives like TNT, RDX, and HMX, due to their manufacturing, testing, and disposal, is becoming a matter of immense interest, worldwide. Most explosives areExpand
Transgenic plants for enhanced phytoremediation of toxic explosives.
  • B. Van Aken
  • Medicine
  • Current opinion in biotechnology
  • 2009
Transgenic plants exhibiting biodegradation capabilities of microorganisms bring the promise of an efficient and environmental-friendly technology for cleaning up polluted soils. Expand


Biodegradation of explosives by transgenic plants expressing pentaerythritol tetranitrate reductase
Transgenic tobacco plants expressing pentaerythritol tetranitrate reductase, an enzyme derived from an explosive-degrading bacterium that enables degradation of nitrate ester and nitroaromatic explosives, suggest that transgenic plants expressing microbial degradative genes may provide a generally applicable strategy for bioremediation of organic pollutants in soil. Expand
Enhanced metabolism of halogenated hydrocarbons in transgenic plants containing mammalian cytochrome P450 2E1.
  • S. Doty, T. Q. Shang, +5 authors M. Gordon
  • Chemistry, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 2000
Transgenic plants with a profound increase in metabolism of the most common contaminant, TCE, are engineered by introducing the mammalian cytochrome P450 2E1 enzyme, which oxidizes a wide range of important pollutants. Expand
Uptake and Transformation of TNT by Hybrid Poplar Trees
This paper examines the potential for using hybrid poplar trees to remediate sites contaminated with the high explosive 2,4,6-trinitrotoluene (TNT). Laboratory experiments assessed the uptake ofExpand
Phytoremediation of methylmercury pollution: merB expression in Arabidopsis thaliana confers resistance to organomercurials.
This work suggests that native macrophytes (e.g., trees, shrubs, grasses) engineered to express merBpe may be used to degrade methylmercury at polluted sites and sequester Hg(II) for later removal. Expand
Uptake of nitroaromatic compounds in plants
Results from soils with a wide range of explosive concentrations show a good correlation between the plant and soil concentrations and it is likely that a metabolic transformation in plants leads to the formation of dinitrotoluenes which are considered to be potent carcinogens. Expand
Transformation of 2,4,6-trinitrotoluene (TNT) by the aquatic plant myriophyllum spicatum
The ability of the aquatic plant Eurasian Watermilfoil (Myriophyllum spicatum) to transform 2,4,6-trinitrotoluene (TNT) was investigated in a series of batch assays. The TNT was added to plantExpand
Confirmation of conjugation processes during TNT metabolism by axenic plant roots
This paper examines processes in plants for the formation of fate products of TNT beyond its aminated reduction products, 2-amino-4,6-dinitrotoluene and 4-amino-2,6-dinitrotoluene. TNT metabolitesExpand
Aerobic Degradation of 2,4,6-Trinitrotoluene byEnterobacter cloacae PB2 and by Pentaerythritol Tetranitrate Reductase
The ability to remove nitrogen from TNT suggests that PB2 or recombinant organisms expressing PETN reductase may be useful for bioremediation of TNT-contaminated soil and water. Expand
Comparison of lead tolerance in Allium cepa with other plant species
The results point to the role played by the storage organ (bulb) in raising the tolerance of A. cepa to lead, which is demonstrated for the first time and interpreted as an element of constitutional tolerance to lead. Expand
Analysis of 2,4,6-trinitrotoluene and its transformation products in soils and plant tissues by high-performance liquid chromatography
Abstract Previous studies have failed to provide an acceptable mass balance for 2,4,6-trinitrotoluene (TNT) in soils and plants due to deficiencies in analytical methodology. A high-performanceExpand