Phytochrome-induced synthesis of ribonuclease de novo in lupin hypocotyl sections.

  title={Phytochrome-induced synthesis of ribonuclease de novo in lupin hypocotyl sections.},
  author={George John Acton and Peter Schopfer},
  journal={The Biochemical journal},
  volume={142 3},
1. Density-labelling with 99 atoms% of (2)H(2)O distinguished pre-existing from newly synthesized ribonuclease molecules in sections of developing hypocotyl tissue. 2. Activity profiles of enzyme extracted from the fraction pelletable at 100000g showed heterogeneity after isopycnic centrifugation in CsCl gradients. 3. Measurement of density shifts of the entire heterogeneous band shows that ribonuclease protein is synthesized de novo in both continuous far-red light and darkness. 4. A twofold… CONTINUE READING

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