Phytochrome from the green alga Mesotaenium caldariorum. Purification and preliminary characterization.

@article{Kidd1990PhytochromeFT,
  title={Phytochrome from the green alga Mesotaenium caldariorum. Purification and preliminary characterization.},
  author={Daniel G. Kidd and John Clark Lagarias},
  journal={The Journal of biological chemistry},
  year={1990},
  volume={265 12},
  pages={7029-35}
}
A method to purify the phytochrome photoreceptor from the unicellular green alga Mesotaenium caldariorum is presented. Preparative scale formation of algal protoplasts and controlled osmotic cell lysis have permitted separation of intact organelles from the phytochrome-enriched soluble protein fraction. We have utilized the observation that red light-absorbing (Pr) and far-red light-absorbing (Pfr) forms of phytochrome are differentially retained on an anion exchange matrix to purify M… CONTINUE READING

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