Phytochrome and blue light-mediated stomatal opening in the orchid, paphiopedilum.

  title={Phytochrome and blue light-mediated stomatal opening in the orchid, paphiopedilum.},
  author={Lawrence D. Talbott and Jianxin Zhu and Seung Won Han and Eduardo Zeiger},
  journal={Plant \& cell physiology},
  volume={43 6},
Guard cells of the orchid genus, Paphiopedilum have been reported to lack developed chloroplasts and detectable chlorophyll a autofluorescence. Paphiopedilum stomata lack a photosynthesis-dependent opening response but have a blue light-specific opening. The present study found that low fluence rate green and red light elicited stomatal opening in Paphiopedilum and this opening was reversed by far red light, indicating the presence of a phytochrome-mediated opening response. Phytochrome… 

Figures from this paper

Slow photosynthetic induction and low photosynthesis in Paphiopedilum armeniacum are related to its lack of guard cell chloroplast and peculiar stomatal anatomy.

The results provide evidence for the morphological and physiological evolution of stomata relation for water conservation under natural selection and reflect an adaptation of Paphiopedilum to periodic water deficiency in limestone habitats.

Blue Light and Phytochrome-Mediated Stomatal Opening in the npq1 and phot1 phot2 Mutants of Arabidopsis1

Blue-green reversibility and far-red light were used to probe the stomatal responses of the npq1 mutant and the phot1 phot2 double mutant of Arabidopsis to demonstrate phytochrome-mediated and blue light-specific responses.

Role of guard cell- or mesophyll cell-localized phytochromes in stomatal responses to blue, red, and far-red light

Guard cell- or mesophyll cell-localized phytochromes do not have a predominant direct light sensory role in red- or blue-light-mediated stomatal opening or far-red-light-mediated stomatal closure of

Phytochrome B is involved in mediating red light-induced stomatal opening in Arabidopsis thaliana.

A positive role for Arabidopsis (Arabidopsis thaliana) phyB is reported in the regulation of red light-induced stomatal opening and quantitative RT-PCR analysis showed that phy B and CRY might regulate stomatic opening, at least in part, by regulating MYB60 expression.

Reversal by green light of blue light-stimulated stomatal opening in intact, attached leaves of Arabidopsis operates only in the potassium-dependent, morning phase of movement.

Previous studies showing that npq1 stomata are devoid of a specific blue light response are confirmed, showing that the shift away from potassium-based osmoregulation in guard cells is further postulated to entail a shift from blue light to photosynthesis as the primary component of the stomatal response to light.

Light-regulated stomatal aperture in Arabidopsis.

A few newly discovered nuclear genes, their function with respect to the phot-, cry-, and phy-mediated signal transduction cascades, and possible involvement of circadian clock are discussed.

Phytochrome B Enhances Photosynthesis at the Expense of Water-Use Efficiency in Arabidopsis1[W][OA]

A model where active phyB promotes stomata differentiation in open places is proposed, allowing plants to take advantage of the higher irradiances at the expense of a reduction of water-use efficiency, which is compensated by a reduced leaf area.

Transcriptome Analysis of Tessellated and Green Leaves in Paphiopedilum Orchids Using Illumina Paired-End Sequencing and Discovery Simple Sequence Repeat Markers

  • D. Li
  • Environmental Science
  • 2015
Paphiopedilum spp, well-known as lady’s slipper orchids in horticulture, belong to Paphiopedilum genus, Orchidaceae family [6]. With respect to leaf traits, Paphiopedilum has coriaceous, green or

The acclimation of Tilia cordata stomatal opening in response to light, and stomatal anatomy to vegetational shade and its components

It was concluded that differences in white light responses between T. cordata leaves grown in different light environments are caused only by their different blue light response, whereas decreased light intensity plays a minor role.




Stomatal opening under red light was indistinguishable from that in darkness, whereas blue light promoted opening above dark levels, suggesting that the previously reported red light responses in stomata from intact Paphiopedilum leaves resulted from indirect effects, such as depletion of intercellular CO2 by mesophyll photosynthesis.

Stomatal Limitation to Carbon Gain in Paphiopedilum sp. (Orchidaceae) and Its Reversal by Blue Light.

Blue light enrichment resulted in significantly higher growth rates-of up to 77%-over a 3 to 4 week growing period, with all evidence indicating that the blue light effect was a stomatal response.

Stomata from npq1, a zeaxanthin-less Arabidopsis mutant, lack a specific response to blue light.

The lack of a specific blue light response in the zeaxanthinless npq1 mutant provides genetic evidence for the role of zeax anthin as a blue light photoreceptor in guard cells.

The inhibitor of zeaxanthin formation, dithiothreitol, inhibits blue-light-stimulated stomatal opening in Vicia faba

The obtained results indicate that zeaxanthin could function as a photoreceptor mediating the stomatal responses to blue light, as well as inhibits blue-light-stimulatedStomatal opening in epidermal peels of Vicia faba L. in a concentration-dependent fashion.

Reversal of blue light-stimulated stomatal opening by green light.

Blue/green reversibility might be explained by a pair of interconvertible zeaxanthin isomers, one absorbing in the blue and the other in the green, with the green absorbing form being the physiologically active one.

Green light reversal of blue-light-stimulated stomatal opening is found in a diversity of plant species.

Results suggest that blue-green reversibility of stomatal opening is a basic photobiological property of guard cells and could provide a valuable diagnostic tool for zeaxanthin-mediated blue-light photoperception.

Metabolic energy for stomatal opening. Roles of photophosphorylation and oxidative phosphorylation

Light-dependent stomatal opening appears to require photophosphorylation from guard-cell chloroplasts and the activation of the blue-light photosystem which could rely either on oxidative phosphorylation or a specific, membrane-bound electron-transport carrier.

Phytochrome involvement in stomatal movement in Pisum sativum, vicia faba and Pelargonium sp.

In leaf pieces of P. sativum blue light was more effective than red, but not in isolated guard cells, and DCMU inhibited stomatal opening in red light more than in blue, and thus increased the relative response to blue light.

Phytochrome is not involved in the red-light-enhancement of the stomatal blue-light-response in wheat seedlings

It is concluded that the red-light-enhancement of the stomatal blue- light-response in wheat seedlings does not involve a change in the photostationary state of phytochrome.