Phytochrome Effects on the Relationship between Chlorophyll and Steady-State Levels of Thylakoid Polypeptides in Light-Grown Tobacco.

Abstract

The effects of phytochrome status on chlorophyll content and on steady-state levels of thylakoid proteins were investigated in green leaves of Nicotiana tabacum L. plants grown under white light. Far-red light given either as a pulse at the end of each photoperiod, or as a supplement to white light during the photoperiod, reduced chlorophyll content per unit area and per unit dry weight. These differences were also observed after resolving chlorophyll-containing polypeptides by gel electrophoresis. Chlorophyll a:b ratio was unchanged. Both Coomassie blue-stained gels and immunochemical analyses showed that, in contrast to the observations in etiolated barley (K Apel, K Kloppstech [1980] Planta 150: 426-430) and pea (J Bennett [1981] Eur J Biochem 118: 61-70) seedlings, and in etiolated tobacco leaves (this report), in fully deetiolated tobacco plants changes in chlorophyll content were not correlated with obvious changes in the steady-state levels of thylakoid proteins (e.g. light-harvesting, chlorophyll a/b-binding proteins).

Cite this paper

@article{Casal1990PhytochromeEO, title={Phytochrome Effects on the Relationship between Chlorophyll and Steady-State Levels of Thylakoid Polypeptides in Light-Grown Tobacco.}, author={Jorge Jos{\'e} Casal and Garry C. Whitelam and Harry Smith}, journal={Plant physiology}, year={1990}, volume={94 1}, pages={370-4} }