The fresh juice from leaves of Sedum dendroideum Moc & Sessé (Crassulaceae) is used in Brazilian traditional medicine for the treatment of gastric and inflammatory disorders. The present investigation was carried out to evaluate in vivo antinociceptive and anti-inflammatory activities of this plant material. The oral administration (0.1-1g/kg) of the lyophilized Sedum dendroideum juice (L J) caused a significant dose-related reduction of acetic acid-induced writhing response (ID(50)=631 mg/kg) and inhibited croton oil-induced ear oedema formation (66% inhibition at 1g/kg) in mice. In the formalin-induced nociception in mice, L J (1g/kg) only inhibited the second phase of nociception (46%). Phytochemical investigation revealed four known kaempferol glycosides, here, described at the first time for this species. These flavonoids probably explain the antinociceptive and anti-inflammatory effects of the fresh juice of Sedum dendroideum.