Phytase activity in tobacco (Nicotiana tabacum) root exudates is exhibited by a purple acid phosphatase.

@article{Lung2008PhytaseAI,
  title={Phytase activity in tobacco (Nicotiana tabacum) root exudates is exhibited by a purple acid phosphatase.},
  author={Shiu-Cheung Lung and Andy Leung and Rainbow Kuang and Yu Wang and Priscilla T Y Leung and Boon-Leong Lim},
  journal={Phytochemistry},
  year={2008},
  volume={69 2},
  pages={365-73}
}
Phytases are enzymes that catalyze liberation of inorganic phosphates from phytate, the major organic phosphorus in soil. Tobacco (Nicotiana tabacum) responds to phosphorus starvation with an increase in extracellular phytase activity. By a three-step purification scheme, a phosphatase with phytase activity was purified 486-fold from tobacco root exudates to a specific activity of 6,028 nkat mg(-1) and an overall yield of 3%. SDS-PAGE revealed a single polypeptide of 64 kDa, thus indicating… CONTINUE READING

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