Physiological responses of the halophilic archaeon Halobacterium sp. strain NRC1 to desiccation and gamma irradiation

@article{Kottemann2005PhysiologicalRO,
  title={Physiological responses of the halophilic archaeon Halobacterium sp. strain NRC1 to desiccation and gamma irradiation},
  author={Molly C. Kottemann and Adrienne Kish and Chika Iloanusi and Sarah J. Bjork and Jocelyne DiRuggiero},
  journal={Extremophiles},
  year={2005},
  volume={9},
  pages={219-227}
}
We report that the halophilic archaeon Halobacterium sp. strain NRC-1 is highly resistant to desiccation, high vacuum and 60Co gamma irradiation. Halobacterium sp. was able to repair extensive double strand DNA breaks (DSBs) in its genomic DNA, produced both by desiccation and by gamma irradiation, within hours of damage induction. We propose that resistance to high vacuum and 60Co gamma irradiation is a consequence of its adaptation to desiccating conditions. Gamma resistance in Halobacterium… 
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175 Citations
Highly Influential Citations
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175 Citations

Extremely Radiation-Resistant Mutants of a Halophilic Archaeon with Increased Single-Stranded DNA-Binding Protein (RPA) Gene Expression
TLDR
It is proposed that RPA facilitates DNA repair machinery and/or protects repair intermediates to maximize the ionizing radiation resistance of this archaeon.
The Survival and Resistance of Halobacterium salinarum NRC-1, Halococcus hamelinensis, and Halococcus morrhuae to Simulated Outer Space Solar Radiation.
TLDR
Overall, this study shows that some halophilic archaea are highly resistant to simulated solar radiation and that they are of high astrobiological significance.
Survival of thermophilic and hyperthermophilic microorganisms after exposure to UV-C, ionizing radiation and desiccation
TLDR
The results show that tolerance to radiation seems to be a common phenomenon among thermophilic and hyperthermophilic microorganisms.
Salt shield: intracellular salts provide cellular protection against ionizing radiation in the halophilic archaeon, Halobacterium salinarum NRC-1.
TLDR
The work presented here supports previous studies showing that radiation resistance is the product of mechanisms for cellular protection and detoxification, as well as for the repair of oxidative damage to cellular macromolecules, and emphasizes the significance of the intracellular milieu in determining microbial radiation resistance.
Survival of the Halophilic Archaeon Halovarius luteus after Desiccation, Simulated Martian UV Radiation and Vacuum in Comparison to Bacillus atrophaeus
TLDR
A novel haloarchaea isolated from Urmia Salt Lake, Iran, Halovarius luteus strain DA50T, was exposed to varying levels of simulated extraterrestrial conditions and compared to that of the bacteria Bacillus atrophaeus, which showed the greatest decrease in viability.
Microarray analysis of the hyperthermophilic archaeon Pyrococcus furiosus exposed to gamma irradiation
TLDR
Surprisingly, the many systems involved in oxygen detoxification and redox homeostasis appeared to be constitutively expressed and several transcriptional regulators and protein kinases highly regulated in response to gamma irradiation were identified.
Effects of intracellular Mn on the radiation resistance of the halophilic archaeon Halobacterium salinarum
TLDR
It is proposed that in H. salinarum, increased tolerance to IR is a combination of metabolic regulatory adjustments and the accumulation of Mn-antioxidant complexes.
Resistance to Ionizing Radiation and Oxidative Stress in Halobacterium salinarum NRC-1
TLDR
The cellular damage and stress response of the extremophile Halobacterium salinarum NRC-1 exposed to chemical oxidants and to ionizing radiation (IR) suggested that cell death resulted from interference with major metabolic pathways rather than generalized oxidative lesions.
Mechanisms of Resistance to Ionizing Radiation in Extremophiles
TLDR
The role of mannosylglycerate, di-myo-inositol phosphate, and trehalose in the IR resistance of various thermophiles are examined and it is concluded the anaerobic environment confers their IR resistance.
Effect of 60Co-Gamma Ionizing Radiation and Desiccation Stress on Protein Profile of Anabaena 7120
TLDR
The data suggests an overlap in proteome changes during recovery against radiation and desiccation stress in Anabaena 7120 cells.
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References

SHOWING 1-10 OF 65 REFERENCES
Protective roles of bacterioruberin and intracellular KCl in the resistance of Halobacterium salinarium against DNA-damaging agents.
TLDR
Results suggest that bacterioruberin and intracellular KCl of H. salinarium protect this organism against the lethal effects of oxidative DNA-damaging agents.
Systems level insights into the stress response to UV radiation in the halophilic archaeon Halobacterium NRC-1.
We report a remarkably high UV-radiation resistance in the extremely halophilic archaeon Halobacterium NRC-1 withstanding up to 110 J/m2 with no loss of viability. Gene knockout analysis in two…
DNA protection mechanisms are not involved in the radioresistance of the hyperthermophilic archaea Pyrococcus abyssi and P. furiosus
TLDR
The linear density of double-stranded breaks was essentially the same in all four microorganisms when irradiation was carried under similar anaerobic conditions, indicating that no specific DNA protection mechanisms exist in Pyrococcus species.
Genome of the Extremely Radiation-Resistant Bacterium Deinococcus radiodurans Viewed from the Perspective of Comparative Genomics
TLDR
An extensive comparative sequence analysis of the Deinococcus genome suggests that several different biological mechanisms contribute to the multiple DNA repair-dependent phenotypes of this organism.
Biopan-survival I: exposure of the osmophiles synechococcus sp. (Nageli) and haloarcula sp. to the space environment
Genome sequence of Halobacterium species NRC-1.
TLDR
Analysis of the genome sequence shows the presence of pathways for uptake and utilization of amino acids, active sodium-proton antiporter and potassium uptake systems, sophisticated photosensory and signal transduction pathways, and DNA replication, transcription, and translation systems resembling more complex eukaryotic organisms.
The effects of ultraviolet radiation on the moderate halophile Halomonas elongata and the extreme halophile Halobacterium salinarum.
TLDR
The purpose of this study was to illustrate the effect that solar (UV-A and UV-B) and germicidal radiation ( UV-C) had on the growth patterns of these bacteria at varied salt concentrations.
Thermococcus gammatolerans sp. nov., a hyperthermophilic archaeon from a deep-sea hydrothermal vent that resists ionizing radiation.
TLDR
It is proposed that this isolate, which constitutes the most radioresistant hyperthermophilic archaeon known to date, should be described as the type strain of a novel species, Thermococcus gammatolerans sp.
The repair of ultraviolet light-induced DNA damage in the halophilic archaebacteria, Halobacterium cutirubrum, Halobacterium halobium and Haloferax volcanii.
In vivo damage and recA-dependent repair of plasmid and chromosomal DNA in the radiation-resistant bacterium Deinococcus radiodurans
TLDR
These studies show that D. radiodurans retains a heterologous plasmid following irradiation and repairs it with the same high efficiency as its chromosomal DNA, while the repair defect in rec30 prevents repair of the plasmids.
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