[Physiological function of myotonin protein kinase].

  • Shoichi Ishiura
  • Published 1997 in
    Nihon rinsho. Japanese journal of clinical…

Abstract

The mutation underlying myotonic dystrophy is the expansion of polymorphic CTG repeat in the 3'-noncoding region of the myotonin protein kinase (MtPK) gene mapping to chromosome 19q13.3. A full-length cDNA of human MtPK was cloned and expressed in COS-1 cells. We purified native full-length MtPK from rat skeletal muscle. This 70 kDa MtPK is localized in sarcoplasmic reticulum fraction, whereas the previously reported 55 kDa protein was observed in nuclear extract or the sarcoplasmic reticulum membrane. Based on the cDNA sequence, human MtPK was previously reported to have two amino acid sequence variations at the C-terminus, one GAARAP (RAP type) and PALPEP (PEP type). The MtPK purified appeared to be almost entirely RAP type. Stable expression of MtPK in mouse C2C12 cells caused the activation of chloride efflux. Expansion of CTG repeats suppressed myogenic differentiation. Collectively, the results indicate that prolonged MtPK activation provides a link between intracellular signal transduction pathway and membrane permeability.

Cite this paper

@article{Ishiura1997PhysiologicalFO, title={[Physiological function of myotonin protein kinase].}, author={Shoichi Ishiura}, journal={Nihon rinsho. Japanese journal of clinical medicine}, year={1997}, volume={55 12}, pages={3200-4} }