Physiological consequences of loss of allosteric activation of yeast NAD+-specific isocitrate dehydrogenase.

@article{Hu2006PhysiologicalCO,
  title={Physiological consequences of loss of allosteric activation of yeast NAD+-specific isocitrate dehydrogenase.},
  author={Gang Hu and Agnes Liau Wei Lin and Lee McAlister-Henn},
  journal={The Journal of biological chemistry},
  year={2006},
  volume={281 25},
  pages={
          16935-42
        }
}
Based on allosteric regulatory properties, NAD+-specific isocitrate dehydrogenase (IDH) is believed to control flux through the tricarboxylic acid cycle in vivo. To distinguish growth phenotypes associated with regulatory dysfunction of this enzyme in Saccharomyces cerevisiae, we analyzed strains expressing well defined mutant forms of IDH or a non-allosteric bacterial NAD+-specific isocitrate dehydrogenase (IDHa). As previously reported, expression of mutant forms of IDH with severe catalytic… CONTINUE READING