Physiological and nutritional determinants of protease secretion by Clostridium sporogenes: characterization of six extracellular proteases

@article{Allison2004PhysiologicalAN,
  title={Physiological and nutritional determinants of protease secretion by Clostridium sporogenes: characterization of six extracellular proteases},
  author={Clive Allison and George Tennant Macfarlane},
  journal={Applied Microbiology and Biotechnology},
  year={2004},
  volume={37},
  pages={152-156}
}
Proteases were the principal secretory proteins of Clostridium sporogenes and were optimally produced after active growth at 37° C. Glucose, ammonia and peptides repressed protease production. Protease formation was maximal in cultures grown at pH 6.5, but proteolytic activity exhibited a pH optimum of 7.0–8.0. Protease activity in culture filtrates was stimulated by divalent metal ions (Ca2+, Mn2+ and Co2+) and was strongly inhibited by ethylene diaminetetraacetate (EDTA) and thimerosal. Non… CONTINUE READING
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