Physiological and biochemical characterization of the two alpha-L-rhamnosidases of Lactobacillus plantarum NCC245.
@article{vila2009PhysiologicalAB, title={Physiological and biochemical characterization of the two alpha-L-rhamnosidases of Lactobacillus plantarum NCC245.}, author={Marta {\'A}vila and Muriel Jaquet and Deborah Moine and Teresa Requena and Carmen Peláez and Fabrizio Arigoni and Ivana Jankovi{\'c}}, journal={Microbiology}, year={2009}, volume={155 Pt 8}, pages={ 2739-49 } }
This work is believed to be the first report on the physiological and biochemical characterization of alpha-l-rhamnosidases in lactic acid bacteria. A total of 216 strains representing 37 species and eight genera of food-grade bacteria were screened for alpha-l-rhamnosidase activity. The majority of positive bacteria (25 out of 35) were Lactobacillus plantarum strains, and activity of the L. plantarum strain NCC245 was examined in more detail. The analysis of alpha-l-rhamnosidase activity under…
78 Citations
L-Rhamnose induction of Aspergillus nidulans α-L-rhamnosidase genes is glucose repressed via a CreA-independent mechanism acting at the level of inducer uptake
- BiologyMicrobial Cell Factories
- 2012
The A. nidulans rhaE gene encodes an α-L-rhamnosidase phylogenetically distant to those described in filamentous fungi, and its expression is regulated by a novel CreA-independent mechanism.
Evaluation and characterization of new α-L-rhamnosidase-producing yeast strains.
- Biology, MedicineThe Journal of general and applied microbiology
- 2015
It was found that these isolates of yeast producing L-rhamnosidase have a great potential application for beverage quality enhancement, and can build a strong foundation of α-L- lusitaniae-producing yeast strains in the debittering of citrus juice.
Comparative genomics and functional analysis of rhamnose catabolic pathways and regulons in bacteria
- BiologyFront. Microbiol.
- 2013
Large-scale regulon inference using bioinformatics revealed remarkable variations in transcriptional regulators for L-Rha utilization genes among bacteria.
Structural and functional insights into RHA-P, a bacterial GH106 α-L-rhamnosidase from Novosphingobium sp. PP1Y.
- BiologyArchives of biochemistry and biophysics
- 2018
The metagenome-derived enzyme RhaB opens a new subclass of bacterial B type α-L-rhamnosidases.
- BiologyJournal of biotechnology
- 2014
Characterization of Two Distinct Glycosyl Hydrolase Family 78 α-l-Rhamnosidases from Pediococcus acidilactici
- BiologyApplied and Environmental Microbiology
- 2011
Detailed kinetic data is presented on two novel rhamnosidases from Pediococcus acidilactici DSM 20284, probably representing two subclasses within GH 78, which could be relevant for the further study of bacterial glycosidases.
Purification and Characterization of α-L-rhamnosidase from Bacillus amyloliquefaciens –D1
- Asian Journal of Biological and Life sciences
- 2021
The effects of mental activity found showed that Fe2+ and NaCl were strong activators, while KCL and Cu2+ was a strong inhibitor of the rhamnosidase enzyme production, which will help in citrus processing and bioprocess industries.
Identification of a novel Penicillium chrysogenum rhamnogalacturonan rhamnohydrolase and the first report of a rhamnogalacturonan rhamnohydrolase gene.
- BiologyEnzyme and microbial technology
- 2017
References
SHOWING 1-10 OF 47 REFERENCES
Molecular identification of an alpha-L-rhamnosidase from Bacillus sp strain GL1 as an enzyme involved in complete metabolism of gellan.
- Biology, EngineeringArchives of biochemistry and biophysics
- 2003
Purification and Characterization of Two Different α-l-Rhamnosidases, RhaA and RhaB, fromAspergillus aculeatus
- Biology, ChemistryApplied and Environmental Microbiology
- 2001
ABSTRACT Two proteins exhibiting α-l-rhamnosidase activity, RhaA and RhaB, were identified upon fractionation and purification of a culture filtrate from Aspergillus aculeatus grown on hesperidin.…
Cloning, sequence analysis, and expression of the gene encoding Sphingomonas paucimobilis FP2001 alpha-L -rhamnosidase.
- Biology, ChemistryCurrent microbiology
- 2005
RT-PCR analysis of rhaM mRNA indicated that the induction of alpha-L-rhamnosidase by the addition of L- rhamnose occurred on the transcriptional level, suggesting that rham is possibly a member of a new bacterial subfamily in glycoside hydrolase family 78 (alpha- L-Rhamnosidsase).
Two new thermostable α-l-rhamnosidases from a novel thermophilic bacterium
- Biology, Engineering
- 2004
Purification and characterization of alpha-L-rhamnosidase from Bacteroides JY-6, a human intestinal bacterium.
- Chemistry, BiologyBiological & pharmaceutical bulletin
- 1996
An alpha-L- rhamnosidase was purified 1500-fold from Bacteroides JY-6, an intestinal anaerobic bacterium of human and was strongly inhibited by L-rhamnose, L-fucose, saccharic acid 1,4-lactone, p-chlormercuriphenylsulfonic acid and Pb2+.
Characterization of alpha-L-rhamnosidase of Bacillus sp. GL1 responsible for the complete depolymerization of gellan.
- Biology, ChemistryArchives of biochemistry and biophysics
- 1999
The alpha-l-rhamnosidase is found to be responsible as the final enzyme for the complete depolymerization of gellan.
The thermostable α‐l‐rhamnosidase RamA of Clostridium stercorarium: biochemical characterization and primary structure of a bacterial α‐l‐rhamnoside hydrolase, a new type of inverting glycoside hydrolase
- Biology, ChemistryMolecular microbiology
- 2000
An α‐l‐rhamnosidase clone was isolated from a genomic library of the thermophilic anaerobic bacterium Clostridium stercorarium and its primary structure was determined, indicating a single displacement mechanism.
Purification and characterization of intracellular α-l-rhamnosidase from Pseudomonas paucimobilis FP2001
- BiologyArchives of Microbiology
- 2000
Examination of the substrate specificity using various synthetic and natural l-rhamnosyl glycosides showed that this enzyme had a relatively broader substrate specificity than those reported so far.
Production of Exopolysaccharide by Lactobacillus rhamnosus R and Analysis of Its Enzymatic Degradation during Prolonged Fermentation
- Medicine, BiologyApplied and Environmental Microbiology
- 2000
The decrease in viscosities and molecular weights of EPS withdrawn at different cultivation times permitted us to suspect the presence of a depolymerizing enzyme in the fermentation medium, and the loss of viscosity was increased up to 33%.
Complete genome sequence of Lactobacillus plantarum WCFS1
- BiologyProceedings of the National Academy of Sciences of the United States of America
- 2003
The 3,308,274-bp sequence of the chromosome of Lactobacillus plantarum strain WCFS1, a single colony isolate of strain NCIMB8826 that was originally isolated from human saliva, has been determined, and contains 3,052 predicted protein-encoding genes, suggesting that these genes form a lifestyle adaptation region in the chromosome.