Physiological and biochemical characteristics of poly gamma-glutamate synthetase complex of Bacillus subtilis.

@article{Ashiuchi2001PhysiologicalAB,
  title={Physiological and biochemical characteristics of poly gamma-glutamate synthetase complex of Bacillus subtilis.},
  author={Makoto Ashiuchi and Chigure Nawa and Tohru Kamei and Jae Jun Song and Seon Pyo Hong and Moon Hee Sung and Kenji Soda and Haruo Misono},
  journal={European journal of biochemistry},
  year={2001},
  volume={268 20},
  pages={5321-8}
}
An enzymatic system for poly gamma-glutamate (PGA) synthesis in Bacillus subtilis, the PgsBCA system, was investigated. The gene-disruption experiment showed that the enzymatic system was the sole machinery of PGA synthesis in B. subtilis. We succeeded in achieving the enzymatic synthesis of elongated PGAs with the cell membrane of the Escherichia coli clone producing PgsBCA in the presence of ATP and D-glutamate. The enzyme preparation solubilized from the membrane with 8 mM Chaps catalyzed… CONTINUE READING

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