Physicochemical studies of biologically active peptides by low-temperature reversed-phase high-performance liquid chromatography.

The high-performance liquid chromatographic separation of biologically active peptides containing 1-prolyl residues is demonstrated. Rapid isomer interconversion between cis- and trans-isomeric forms at the prolyl peptide bond is shown to produce classical secondary equilibrium effects in the peak shapes. By operating the column at temperatures in the range… CONTINUE READING