Physicochemical and biological properties of mycobacteriocin M12 produced by Mycobacterium smegmatis ATCC 25855.

Abstract

A mycobacteriocin (M12) produced by Mycobacterium smegmatic ATCC 25855 was partially purified by ammonium sulphate precipitation followed by DEAE-cellulose chromatography and Sephadex G100 chromatography. Production of M12 was maximal when bacteria were harvested after 3 d cultivation in liquid medium and disrupted by sonication. The molecular weight of M12… (More)

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