Physico-chemical factors governing partition behaviour of solutes and particles in aqueous polymeric biphasic systems. III. Features of solutes and biological particles detected by the partition technique.

@article{BYu1983PhysicochemicalFG,
  title={Physico-chemical factors governing partition behaviour of solutes and particles in aqueous polymeric biphasic systems. III. Features of solutes and biological particles detected by the partition technique.},
  author={Zaslavsky BYu and N M Mestechkina and L M Miheeva and Sergei V. Rogozhin},
  journal={Journal of chromatography},
  year={1983},
  volume={256 1},
  pages={49-59}
}
The partition behaviour of human serum albumin and oxyhaemoglobin and several amino acids and small peptides was studied in the aqueous Ficoll-dextran biphasic system as a function of the ionic composition and pH. The partition coefficients of the solutes were expressed in terms of the equivalent number of CH2 groups, nCH2, and the equivalent number of carboxyl groups, m. The physical meaning of these two parameters and of the relationships found between them and pH for the proteins examined… CONTINUE READING

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