Physical organization of the upper pathway operon promoter of the Pseudomonas TOL plasmid. Sequence and positional requirements for XylR-dependent activation of transcription

@article{Abril1993PhysicalOO,
  title={Physical organization of the upper pathway operon promoter of the Pseudomonas TOL plasmid. Sequence and positional requirements for XylR-dependent activation of transcription},
  author={Mauricio Abril and Juan L. Ramos},
  journal={Molecular and General Genetics MGG},
  year={1993},
  volume={239},
  pages={281-288}
}
The upper pathway operon of the Pseudomonas putida TOL plasmid belongs to the −12/-24 class of promoters. These promoters exhibit three regions critical for regulated transcription, namely, the −12/−24 site for RNA polymerase/σ54 binding, the −55/−67 region for IHF protein binding, and the -130(UAS2)/−170(UAS1) region, where two sites for XylR binding are located. The XylR-protected G residues located at −131, −139, −160 and −169 were replaced with As, and the activity of the mutant promoters… CONTINUE READING

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Role of integration host factor in the regulation of the glnHp2 promoter of Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America • 1991
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