Physical mapping of the CXC chemokine locus on human chromosome 4

  title={Physical mapping of the CXC chemokine locus on human chromosome 4},
  author={N. O’Donovan and Matthew Galvin and John G. Morgan},
  journal={Cytogenetic and Genome Research},
  pages={39 - 42}
A physical map of the CXC chemokine locus on chromosome 4 has been constructed by PCR analysis and PFGE mapping of YAC clones. The genes for IL8, GRO1, PPBP, PF4, SCYB5 (ENA-78) and SCYB6 (GCP-2) have been co-localized on a 335-kb genomic fragment. The GRO2 and GRO3 genes did not map within this region and based on analysis of a YAC contig overlapping IL8 we speculate that GRO2 and GRO3 map downstream of this region. We have also assigned the novel CXC chemokine gene, SCYB9B (alias H174/βR1) to… 

Figures and Tables from this paper

High-resolution mapping of the human 4q21 and the mouse 5E3 SCYB chemokine cluster by fiber-fluorescence in situ hybridization

The results refine previous maps of the three genes, support the hypothesis that they resulted from gene duplication that took place in a common ancestor of mouse and human, and provide complementary information on a region of the draft sequence of human Chr 4 that is not yet covered.

Cloning and genomic localization of the murine LPS-induced CXC chemokine (LIX) gene, Scyb5

The sequence and mapping data suggest that the human ENA78-PBP-PF4 and GCP2-ψPBP -PF4V1 loci arose from an evolutionarily recent duplication of an ancestral locus related to the murine Lix-Pbp-Pf4 locus.

Organization of the chemokine genes in the human and mouse major clusters of CC and CXC chemokines: diversification between the two species

The results provide an important insight into the evolutionary processes that generated the major chemokine gene clusters and also valuable information in assigning the orthologues between human and mouse major cluster chemokines.

Identification, mapping, and phylogenetic analysis of three novel chicken CC chemokines

Three novel chicken CC chemokine genes among cDNA clones derived from lipopolysaccharide-stimulated cells of the chicken macrophage cell line HD11 suggest that the complex organization of the immune system and diversity of responding cells were largely in place at that time.

Cloning, genomic sequence, and chromosome mapping of Scyb11, the murine homologue of SCYB11 (alias βR1/H174/SCYB9B/I-TAC/IP-9/CXCL11)

The cDNA of the murine homologue of this protein, Scyb11, is cloned from interferon-γ/lipopolysaccharide-stimulated RAW264.7 mouse macrophage-like cells and confirms the mouse/human homology of the two chromosome regions.

Localization of distal regulatory domains in the megakaryocyte-specific platelet basic protein/platelet factor 4 gene locus.

Examination of PBP and PF4 expression in transgenic mice using 4 distinct human PBP/PF4 gene locus constructs showed that within the region studied there was sufficient information to regulate tissue-specific expression of both hPBP and hPF4, and it was indicated that the DNA domains that led to this expression were distinct for the 2 genes.

Application of Comparative Transcriptional Genomics to Identify Molecular Targets for Pediatric IBD

This study provides a comprehensive view of transcriptome changes between different pediatric IBD populations in comparison with different colitis models, and reveals several new molecular targets for further study in the regulation of colitis.

The Murine Chemokine CXCL11 (IFN-Inducible T Cell α Chemoattractant) Is an IFN-γ- and Lipopolysaccharide- Inducible Glucocorticoid-Attenuated Response Gene Expressed in Lung and Other Tissues During Endotoxemia1

The structural and regulatory similarities of murine and human I-TAC suggest that mouse models will be useful for investigating the role of this chemokine in human biology and disease.

p38 Mitogen-Activated Protein Kinase-Dependent and -Independent Signaling of mRNA Stability of AU-Rich Element-Containing Transcripts

The identification of the p38-dependent upstream activator MAP kinase kinase 6 as a member of this group identifies a positive feedback loop regulating macrophage signaling via p38MAP kinase-dependent transcript stabilization.



Localization of the gene for the human MIG cytokine on chromosome 4q21 adjacent to INP10 reveals a chemokine "mini-cluster".

It is demonstrated by pulsed field gel electrophoresis that MIG and INP10 are distant from the tight cluster of other CXC chemokine genes at 4q12-->q13, and determined that Mig and INp10 are oriented head to tail with their start codons separated by less than 16 kb.

Identification of three related human GRO genes encoding cytokine functions.

  • S. HaskillA. Peace R. Sager
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1990
expression studies reveal tissue-specific regulation as well as regulation by specific inducing agents, including interleukin 1, tumor necrosis factor, phorbol 12-myristate 13-acetate, and lipopolysaccharide, suggesting a role in regulation.

Genes for beta-thromboglobulin and platelet factor 4 are closely linked and form part of a cluster of related genes on chromosome 4.

It is shown that the beta TG genes are closely linked to the PF4 genes and to other previously mapped CXC SIGs, namely IL8, GRO1, and two related genes GRO2 and GRO3, on a single 700-kb Sfil fragment localized to chromosome bands 4q12-q13.

Interferon-inducible gene maps to a chromosomal band associated with a (4;11) translocation in acute leukemia cells.

The results suggest a model in which juxtaposition of genetic loci regulated by antiproliferative signals, such as interferon, next to an oncogene, like ETS1, could effectively short circuit homeostatic control circuits and contribute to the neoplastic state.

Physical mapping of chromosome 8p22 markers and their homozygous deletion in a metastatic prostate cancer.

Physical mapping places cosmid CI8-2644 telomeric to MSR (macrophage scavenger receptor), the reverse of a previously published map, altering the interpretation of published deletion studies.

Sequence similarities of a subgroup of CXC chemokines related to murine LIX: implications for the interpretation of evolutionary relationships among chemokines

Phylogenetic analysis shows that the LIX coding region is more distant from both human GCP‐2 and ENA‐78 than is porcine AMCF‐II, the chemokine with greatest sequence similarity to LIX, which shows that a one‐to‐one genetic correspondence does not necessarily exist between all theChemokine genes in two different species.

Molecular characterization and chromosomal mapping of melanoma growth stimulatory activity, a growth factor structurally related to beta‐thromboglobulin.

The level of MGSA mRNA in melanoma cells is strongly elevated by treatment with MGSA, and the gene product of a recently detected gene gro was mapped to chromosome 4 (region q13––q21).

Cloning and characterization of the human neutrophil-activating peptide (ENA-78) gene.

Structure and chromosomal localization of the human stromal cell-derived factor 1 (SDF1) gene.

Stromal cell-derived factors 1 alpha and 1 beta are small cytokines belonging to the intercrine CXC subfamily and originally isolated from a murine bone-marrow stroma cell line by the signal sequence trap method, and strong evolutionary conservation and unique chromosomal localization of the SDF1 gene suggest that they may have important functions distinct from those of other members of the interCrine family.

Mapping of the locus for autosomal dominant amelogenesis imperfecta (AIH2) to a 4-Mb YAC contig on chromosome 4q11-q21.

Amelogenesis imperfecta (AI) is a clinically and genetically heterogeneous group of inherited enamel defects. We recently mapped a locus for autosomal dominant local hypoplastic amelogenesis