Physical mapping of an origin of bidirectional replication at the centre of the Borrelia burgdorferi linear chromosome.

@article{Picardeau1999PhysicalMO,
  title={Physical mapping of an origin of bidirectional replication at the centre of the Borrelia burgdorferi linear chromosome.},
  author={Mathieu Picardeau and J. R. Lobry and B Joseph Hinnebusch},
  journal={Molecular microbiology},
  year={1999},
  volume={32 2},
  pages={437-45}
}
The Borrelia burgdorferi chromosome is linear, with telomeres characterized by terminal inverted repeats and covalently closed single-stranded hairpin loops. The replication mechanism of these unusual molecules is unknown. Previous analyses of bacterial chromosomes for which the complete sequence has been determined, including that of B. burgdorferi, revealed an abrupt switch in polarity of CG skew at known or putative origins of replication. We used nascent DNA strand analysis to physically… CONTINUE READING