Physical map of the Listeria monocytogenes chromosome.

  title={Physical map of the Listeria monocytogenes chromosome.},
  author={Eric J Michel and Pascale Cossart},
  journal={Journal of bacteriology},
  volume={174 22},
The circular physical map of the pathogenic bacterium Listeria monocytogenes LO28 (serovar 1/2c) was established by using pulsed-field gel electrophoresis. The L. monocytogenes chromosome contains eight NotI fragments of 1,100, 940, 400, 335, 280, 45, 30, and 20 kb in size and eight Sse8387I fragments of 860, 680, 680, 370, 335, 130, 70, and 25 kb. Therefore, the total length of the genome is 3,150 kb. To order the NotI fragments on the chromosome, we used a strategy which can be of general use… CONTINUE READING


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