Physical map of the 3' region of the human immunoglobulin heavy chain locus: clustering of autoantibody-related variable segments in one haplotype.

@article{Shin1991PhysicalMO,
  title={Physical map of the 3' region of the human immunoglobulin heavy chain locus: clustering of autoantibody-related variable segments in one haplotype.},
  author={Euy Kyun Shin and Fumihiko Matsuda and Hitoshi Nagaoka and Yosho Fukita and Tsuneo Imai and Katsushi Yokoyama and Eiichi Soeda and T. Honjo},
  journal={The EMBO journal},
  year={1991},
  volume={10 12},
  pages={3641-5}
}
We have constructed the physical map of the 3' region of the human immunoglobulin heavy chain variable region (VH) genes. DNA segments extending to 200 kb upstream of the JH segment were isolated in two YAC clones. Five VH segments were identified in this region in the 5' to 3' order, V(II-5), V(IV-4), V(I-3), V(I-2), and V(VI-1) segments which were all structurally normal and orientated in the same direction as the JH segments. From DNA of a different cell line we have isolated a cosmid contig… CONTINUE READING