Physical association between MHC class I and class II molecules detected on the cell surface by flow cytometric energy transfer.

@article{Szllsi1989PhysicalAB,
  title={Physical association between MHC class I and class II molecules detected on the cell surface by flow cytometric energy transfer.},
  author={J{\'a}nos Sz{\"o}ll{\"o}si and S{\'a}ndor Damjanovich and Margit Bal{\'a}zs and P{\'e}ter Nagy and Lajos Tr{\'o}n and Mack J Fulwyler and Frances M. Brodsky},
  journal={Journal of immunology},
  year={1989},
  volume={143 1},
  pages={208-13}
}
The physical association of HLA class I and class II Ag in the membranes of PGF and JY lymphoblastoid cell lines was studied using flow cytometric energy transfer. This technique measures the proximity of cell surface molecules in the nm range and provides a distribution histogram of the average proximity of molecules on each cell of a population. HLA Ag were labeled with mAb conjugated to fluorescein, serving as donor, or tetramethylrhodamine, serving as acceptor molecules. Significant… CONTINUE READING