Physical and genetic mapping of mammalian replication origins.

@article{Dijkwel1999PhysicalAG,
  title={Physical and genetic mapping of mammalian replication origins.},
  author={Pieter A. Dijkwel and Joyce L. Hamlin},
  journal={Methods},
  year={1999},
  volume={18 3},
  pages={418-31}
}
The neutral/neutral and neutral/alkaline two-dimensional gel electrophoretic techniques are sensitive physical mapping methods that have been used successfully to identify replication initiation sites in genomes of widely varying complexity. We present detailed methodology for the preparation of replication intermediates from mammalian cells and their analysis by both neutral/neutral and neutral/alkaline two-dimensional gel approaches. The methods described allow characterization of the… CONTINUE READING