Physical and genetic-interaction density reveals functional organization and informs significance cutoffs in genome-wide screens.

@article{Dittmar2013PhysicalAG,
  title={Physical and genetic-interaction density reveals functional organization and informs significance cutoffs in genome-wide screens.},
  author={John C. Dittmar and Steven Pierce and Rodney Rothstein and Robert J. D. Reid},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2013},
  volume={110 18},
  pages={7389-94}
}
Genome-wide experiments often measure quantitative differences between treated and untreated cells to identify affected strains. For these studies, statistical models are typically used to determine significance cutoffs. We developed a method termed "CLIK" (Cutoff Linked to Interaction Knowledge) that overlays biological knowledge from the interactome on screen results to derive a cutoff. The method takes advantage of the fact that groups of functionally related interacting genes often respond… CONTINUE READING