Physical and gene mapping of cauliflower (Brassica oleracea) mitochondrial DNA

  title={Physical and gene mapping of cauliflower (Brassica oleracea) mitochondrial DNA},
  author={Philippe Ch{\'e}tritl and Chantal Mathieu and Jean Pierre Muller and Fernand Vedel},
  journal={Current Genetics},
A physical map of the mitochondrial DNA isolated from B. oleracea (cauliflower) inflorescences was constructed with the restriction endonucleases Sall, Kpnl and Bgll. Physical mapping was made using the multi enzyme method with either unlabeled or labeled DNA fragments isolated by preparative electrophoresis and a clone bank prepared by inserting incomplete Sall restriction digests of mitochondrial DNA into a cosmid vector. The different mapping studies led to a circular map, about 217 kb in… CONTINUE READING


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