Physical and functional characterization of the ColS8 plasmid.

  title={Physical and functional characterization of the ColS8 plasmid.},
  author={Marta E Garcia and Keith G. H. Dyke},
  volume={124 1},
The restriction map of the 5.1-kb colicinogenic plasmid ColS8 is reported. Transposon-insertion mutagenesis has been carried out to investigate the location of the various functional regions of this plasmid. Twenty-six independent ColS8::Tn1 insertions and six different deletion mutant plasmids were isolated, and the locations of the insertions and deletions were determined. The mapping of the transposon-insertion sites, together with characterization of the phenotypes of these mutants… CONTINUE READING

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