Physical and biochemical characterization of recombination-dependent synthesis of linear plasmid multimers in Bacillus subtilis.

@article{Leonhardt1991PhysicalAB,
  title={Physical and biochemical characterization of recombination-dependent synthesis of linear plasmid multimers in Bacillus subtilis.},
  author={Heinrich Leonhardt and R. Lurz and Juan Alonso},
  journal={Nucleic acids research},
  year={1991},
  volume={19 3},
  pages={497-503}
}
The synthesis and structure of linear multimeric plasmid molecules (hmw DNA) in Bacillus subtilis were investigated. The replication of covalently-closed-circular supercoiled (form I) DNA requires the rate-limiting plasmid-encoded replication initiation protein. Unlike form I, hmw DNA synthesis is partially resistant to inhibition of cellular transcription… CONTINUE READING