Physical and biochemical characterization of a purified arginyl-tRNA synthetase-lysyl-tRNA-synthetase complex from rat liver.

@article{Dang1982PhysicalAB,
  title={Physical and biochemical characterization of a purified arginyl-tRNA synthetase-lysyl-tRNA-synthetase complex from rat liver.},
  author={C Van Dang and R L Glinski and P C Gainey and Richard H Hilderman},
  journal={Biochemistry},
  year={1982},
  volume={21 8},
  pages={1959-66}
}
Arginyl- and lysyl-tRNA synthetases copurify throughout a six-step chromatographic procedure resulting in a purification of 605- and 559-fold, respectively. The purified enzymes were estimated to be 98% pure with a stoichiometry of 1:1 from acrylamide gel electrophoresis under denaturing conditions. On the basis of a native molecular weight of 285000… CONTINUE READING