Physical Limits on the Mechanical Measurement of the Secondary Structure of Biomolecules

The forces and lengths needed to mechanically denature a protein or nucleic acid are within the capabilities of the Atomic-Force Microscope. However, thermal fluctuations in the filament between the partially unfolded molecule and the force cantilever attenuate any measure of secondary structure on a length ( 2 / n 2 ) ( e / k B T ) 3 / 2 (U I… CONTINUE READING