Physarum amoebae express a distinct fragmin-like actin-binding protein that controls in vitro phosphorylation of actin by the actin-fragmin kinase.

@article{Tjampens1999PhysarumAE,
  title={Physarum amoebae express a distinct fragmin-like actin-binding protein that controls in vitro phosphorylation of actin by the actin-fragmin kinase.},
  author={Davy T'jampens and Juliet Bailey and Lynnette J. Cook and Bara Constantin and Jo{\"e}l Vandekerckhove and Jan Gettemans},
  journal={European journal of biochemistry},
  year={1999},
  volume={265 1},
  pages={240-50}
}
Amoebae and plasmodia constitute the two vegetative growth phases of the Myxomycete Physarum. In vitro and in vivo phosphorylation of actin in plasmodia is tightly controlled by fragmin P, a plasmodium-specific actin-binding protein that enables actin phosphorylation by the actin-fragmin kinase. We investigated whether amoebal actin is phosphorylated by this kinase, in spite of the lack of fragmin P. Strong actin phosphorylation was detected only following addition of recombinant actin-fragmin… CONTINUE READING