Phylogenomics using formalin-fixed and 100+ year-old intractable natural history specimens.

@article{Ruane2017PhylogenomicsUF,
  title={Phylogenomics using formalin-fixed and 100+ year-old intractable natural history specimens.},
  author={Sara Ruane and Christopher C Austin},
  journal={Molecular ecology resources},
  year={2017},
  volume={17 5},
  pages={1003-1008}
}
Museum specimens provide a wealth of information to biologists, but obtaining genetic data from formalin-fixed and fluid-preserved specimens remains challenging. While DNA sequences have been recovered from such specimens, most approaches are time-consuming and produce low data quality and quantity. Here, we use a modified DNA extraction protocol combined with high-throughput sequencing to recover DNA from formalin-fixed and fluid-preserved snakes that were collected over a century ago and for… CONTINUE READING
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