Phylogenetically distant barley legumains have a role in both seed and vegetative tissues.

@article{Julin2013PhylogeneticallyDB,
  title={Phylogenetically distant barley legumains have a role in both seed and vegetative tissues.},
  author={Israel Juli{\'a}n and Jacinto Gandullo and Ludier Kesser Santos-Silva and Isabel D{\'i}az and Manuel Mart{\'i}nez},
  journal={Journal of experimental botany},
  year={2013},
  volume={64 10},
  pages={
          2929-41
        }
}
Legumains or vacuolar processing enzymes are cysteine peptidases (C13 family, clan CD) with increasingly recognized physiological significance in plants. They have previously been classified as seed and vegetative legumains. In this work, the entire barley legumain family is described. The eight members of this family belong to the two phylogenetic clades in which the angiosperm legumains are distributed. An in-depth molecular and functional characterization of a barley legumain from each group… 
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Genome-wide identification and comprehensive gene expression pattern analysis of papain- like and legumain-like proteases in rice suggests their multiple and cooperative roles in seed development and response to environmental variations, which provides several useful cues for further in-depth study.
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The development of novel techniques based on shotgun proteomics, activity-based protein profiling, and comparative and structural genomics will help to achieve a general view of the proteolytic process during germination.
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TLDR
The wide variability in gluten-degrading capacities showed highlights the possibility to select the protease with the highest potential to alter grain composition reducing the gluten content, and new avenues could be explored combining genetic manipulation of proteolytic processes with silencing techniques to be used as biotechnological tools against gluten-related disorders.
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TLDR
Down‐regulation of VPE activity through nematode effectors promotes the nematodes invasion rates and leads to avoidance of the induction of the plant proteolytic response and death of the invading juveniles.
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