Phylogenetic relationships in Nicotiana (Solanaceae) inferred from multiple plastid DNA regions.

@article{Clarkson2004PhylogeneticRI,
  title={Phylogenetic relationships in Nicotiana (Solanaceae) inferred from multiple plastid DNA regions.},
  author={James Clarkson and Sandra Knapp and Vicente F. Garcia and Richard G. Olmstead and Andrew R Leitch and Mark W. Chase},
  journal={Molecular phylogenetics and evolution},
  year={2004},
  volume={33 1},
  pages={
          75-90
        }
}
For Nicotiana, with 75 naturally occurring species (40 diploids and 35 allopolyploids), we produced 4656bp of plastid DNA sequence for 87 accessions and various outgroups. The loci sequenced were trnL intron and trnL-F spacer, trnS-G spacer and two genes, ndhF and matK. Parsimony and Bayesian analyses yielded identical relationships for the diploids, and these are consistent with other data, producing the best-supported phylogenetic assessment currently available for the genus. For the… CONTINUE READING
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