Phylogenetic position of the spirochetal genus Cristispira.

  title={Phylogenetic position of the spirochetal genus Cristispira.},
  author={Bruce J Paster and Dale A. Pelletier and Floyd E. Dewhirst and William G. Weisburg and Vivian Fussing and Lars K Poulsen and Sonja Dannenberg and Imke Schr{\"o}der},
  journal={Applied and environmental microbiology},
  volume={62 3},
Comparative sequence analysis of 16S rRNA genes was used to determine the phylogenetic relationship of the genus Cristispira to other spirochetes. Since Cristispira organisms cannot presently be grown in vitro, 16S rRNA genes were amplified directly from bacterial DNA isolated from Cristispira cell-laden crystalline styles of the oyster Crassostrea virginica. The amplified products were then cloned into Escherichia coli plasmids. Sequence comparisons of the gene coding for 16S rRNA (rDNA… CONTINUE READING
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