Phylogenetic inferences on the relationship of North American and European Picea species based on nuclear ribosomal 18S sequences and the internal transcribed spacer 1 region.

Abstract

We have used PCR (polymerase chain reaction) to isolate nuclear ribosomal sequences from seven species of Picea (spruce). The amplified products contained approximately 200 base pairs from the 3' end of the 18S rRNA gene and the entire first internal transcribed spacer (ITS1). The sequences from these two functionally distinct regions were aligned and… (More)

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