Phylogenetic identification of two major nitrogen-fixing bacteria associated with sugarcane.

@article{Sievers1998PhylogeneticIO,
  title={Phylogenetic identification of two major nitrogen-fixing bacteria associated with sugarcane.},
  author={Martin Sievers and Hans G{\"u}nter Schlegel and J{\'e}sus Caballero-Mellado and Johanna D{\"o}bereiner and Wolfgang Ludwig},
  journal={Systematic and applied microbiology},
  year={1998},
  volume={21 4},
  pages={505-8}
}
Acetobacter diazotrophicus and Herbaspirillum seropedicae were identified by genetic methods based on 16S rRNA sequences. A specific PCR method in combination with probing was developed for A. diazotrophicus. The PCR system includes four primers, of which the primers named AC (CTGTTTCCCGCAAGGGAC) and DI (GCGCCCCATTGCTGGGTT) generated an 445 bp amplicon in all of the 11 A. diazotrophicus strains tested. The phylogenetic position of H. seropedicae was determined. H. seropedicae forms with… CONTINUE READING

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