Phylogenetic analysis guided by intragenomic SSU rDNA polymorphism refines classification of “Alexandrium tamarense” species complex

@inproceedings{Miranda2012PhylogeneticAG,
  title={Phylogenetic analysis guided by intragenomic SSU rDNA polymorphism refines classification of “Alexandrium tamarense” species complex},
  author={Luis Alberto Escudero Miranda and Yunyun Zhuang and Huan Zhang and Senjie Lin},
  year={2012}
}
Abstract We analyzed small subunit ribosomal rRNA gene (SSU rDNA) in single-cell isolated strains of “ Alexandrium tamarense ”, “ Alexandrium fundyense ” and “ Alexandrium catenella ” (Atama complex) by both direct and clone-based sequencing, and found 42–50 intragenomic SSU rDNA polymorphic sites (High IRP) in some of the strains but none or one (No/Low IRP) in others. Clone sequencing of the High-IRP amplicons revealed numerous variants with 0–3.5% nucleotide differences. Phylogenetic… CONTINUE READING

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