Phylogenetic analyses of ribosomal DNA-containing bacterioplankton genome fragments from a 4000 m vertical profile in the North Pacific Subtropical Gyre.

@article{Pham2008PhylogeneticAO,
  title={Phylogenetic analyses of ribosomal DNA-containing bacterioplankton genome fragments from a 4000 m vertical profile in the North Pacific Subtropical Gyre.},
  author={Vinh D Pham and Konstantinos T. Konstantinidis and Tsultrim Palden and Edward F DeLong},
  journal={Environmental microbiology},
  year={2008},
  volume={10 9},
  pages={2313-30}
}
High-throughput identification of rRNA gene-containing clones in large insert metagenomic libraries is difficult, because of the high background of host ribosomal RNA (rRNA) and rRNA genes. To address this challenge, a membrane hybridization method was developed to identify all bacterial small subunit rRNA-containing fosmid clones of microbial community DNA from seven different depths in the North Pacific Subtropical Gyre. Out of 101,376 clones screened, 751 rDNA-containing clones were… CONTINUE READING

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