Phylogenetic Relationships of Basal Hexapods Reconstructed from Nearly Complete 18S and 28S rRNA Gene Sequences

  title={Phylogenetic Relationships of Basal Hexapods Reconstructed from Nearly Complete 18S and 28S rRNA Gene Sequences},
  author={Yan Gao and Y. Bu and Yun‐Xia Luan},
  booktitle={Zoological science},
Abstract This study combined nearly complete 28S and 18S rRNA gene sequences (>4100 nt long) to investigate the phylogenetic relationships of basal hexapods (Protura, Collembola, and Diplura). It sequenced more 28S genes, to expand on a previous study from this lab that used 18S plus only a tiny part of the 28S gene. Sixteen species of basal hexapods, five insects, six crustaceans, two myriapods, and two chelicerates were included in the analyses. Trees were constructed with maximum likelihood… 
Molecular phylogenetic analyses support the monophyly of Hexapoda and suggest the paraphyly of Entognatha
This is the first study to include all hexapod orders in a phylogenetic analysis using multiple nuclear protein-coding genes to investigate the phylogeny of Hexapoda, with an emphasis on Entognatha, and shows that Protura is the sister lineage to all other hexapods and that DiplURA or Diplura + Collembola is closely related to Ectognatha.
Comparative Analysis of Mitochondrial Genomes in Diplura (Hexapoda, Arthropoda): Taxon Sampling Is Crucial for Phylogenetic Inferences
Partitioned phylogenetic analyses retrieve significant support for a monophyletic Diplura within Pancrustacea, with Projapygoidea more closely related to Campodeoidea than to Japygoidesa, which explains the dipluran polyphyly found by past mitogenomic studies.
Phylomitogenomic analyses on collembolan higher taxa with enhanced taxon sampling and discussion on method selection
It is shown that mitochondrial genomes could provide important information for reconstructing the relationships among Collembola when suitable analytical approaches are implemented and the combination of nucleotide sequences, partitioning model and exclusion of third codon positions performed better in generating more reliable tree topology and higher node supports than others.
Re-Evaluating the Internal Phylogenetic Relationships of Collembola by Means of Mitogenome Data
Two new Antarctic Collembola mitogenomes are described and all complete or semi-complete springtail mitogenome sequences available on GenBank are compared in terms of both gene order and DNA sequence analyses in a genome evolution and molecular phylogenetic framework.
Going Deeper into High and Low Phylogenetic Relationships of Protura
Mitochondrial and nuclear markers have been applied herein to investigate and summarize proturan systematics at different hierarchical levels and most morphologically described species were confirmed using molecular markers, with some exceptions, and the advantages of including nuclear, as well as mitochondrial, markers and morphology are discussed.
The Complete Mitochondrial Genomes of Three Bristletails (Insecta: Archaeognatha): The Paraphyly of Machilidae and Insights into Archaeognathan Phylogeny
The gene orders were identical to that of Drosophila yakuba and most bristletail species suggesting a conserved genome evolution within the Archaeognatha, and the relationships among archaeognathan subfamilies seemed to be tangled.
Unexpected diversity in Neelipleona revealed by molecular phylogeny approach ( Hexapoda , Collembola )
The interpretation of the phylogenetic pattern and number of transformations (branch length) indicates that Neelipleona are more diverse than previously thought, with probably many species yet to be discovered.
Mitochondrial Genome Diversity in Collembola: Phylogeny, Dating and Gene Order
This study sequence two new mitochondrial genomes and reanalyze all known Collembola mt-genomes to produce an improved phylogenetic hypothesis for the group, develop a tentative time frame for their differentiation, and provide a comprehensive overview of gene order diversity.
MicroRNA evolution provides new evidence for a close relationship of Diplura to Insecta
This study identified 91 conserved microRNA families from 36 panarthropod taxa, including seven newly sequenced non‐insect hexapods, and found major clade differentiation accompanied by the origin of novel miRNA families, and most miRNA clusters are conserved with a high degree of microsynteny.


The phylogenetic positions of three Basal-hexapod groups (protura, diplura, and collembola) based on ribosomal RNA gene sequences.
This study combined complete 18S with partial 28S ribosomal RNA gene sequences to investigate the relations of basal hexapods, and found data are consistent with hexapod monophyly and Collembola grouped weakly with "Protura + Diplura" under most analytical conditions.
Ribosomal DNA gene and phylogenetic relationships of Diplura and lower Hexapods
Dplura had a close phylogenetic relationship to Protura with high bootstrap support, and the integrated data of 18SrDNA and 28Sr DNA could provide better phylogenetic information, which well supported the monophyly of Diplura.
Revealing pancrustacean relationships: Phylogenetic analysis of ribosomal protein genes places Collembola (springtails) in a monophyletic Hexapoda and reinforces the discrepancy between mitochondrial and nuclear DNA markers
The analyses clearly suggest that Hexapoda is monophyletic, which underpins the inconsistency between nuclear and mitochondrial datasets when analyzing pancrustacean relationships.
28S and 18S rDNA sequences support the monophyly of lampreys and hagfishes.
Moderate to very strong support is provided for the monophyly of the cyclostomes in lampreys plus hagfishes and the currently accepted hypothesis of a lamprey-gnathostome clade is moderately rejected by the Kishino-Hasegawa test and resoundingly rejected by parametric bootstrap tests.
Further use of nearly complete 28S and 18S rRNA genes to classify Ecdysozoa: 37 more arthropods and a kinorhynch.
Phylogenetic analysis of mitochondrial protein coding genes confirms the reciprocal paraphyly of Hexapoda and Crustacea
The finding of the reciprocal paraphyly of Hexapoda and Crustacea suggests an evolutionary scenario in which the acquisition of the hexapod condition may have occurred several times independently in lineages descending from different crustacean-like ancestors, possibly as a consequence of the process of terrestrialization.
The position of crustaceans within Arthropoda - Evidence from nine molecular loci and morphology
The monophyly of Crustacea, relationships of crustaceans to other arthropods, and internal phylogeny of Crustacea are appraised via parsimony analysis in a total evidence framework. Data include
The Phylogeny of the Extant Hexapod Orders
A well‐resolved and robust cladogram of ordinal relationships is produced with the topology of crustacea.
Phylogenetic analysis of arthropods using two nuclear protein–encoding genes supports a crustacean + hexapod clade
  • J. Shultz, J. C. Regier
  • Biology
    Proceedings of the Royal Society of London. Series B: Biological Sciences
  • 2000
Results from maximum–parsimony and maximum–likelihood analyses strongly supported the existence of a Crustacea+ Hexapoda clade (Pancrustacea) over a Myriapoda + Hexapod clade(s) (Atelocerata) and the apparent incompatibility between the molecule–based Pancrustacea hypothesis and morphology–based AtelOCerata hypothesis is discussed.