Phylogenetic Position of the Japanese River Otter Lutra nippon Inferred from the Nucleotide Sequence of 224 bp of the Mitochondrial Cytochrome b Gene

  title={Phylogenetic Position of the Japanese River Otter Lutra nippon Inferred from the Nucleotide Sequence of 224 bp of the Mitochondrial Cytochrome b Gene},
  author={T. Suzuki and Hajime J Yuasa and Yoshiyasu Machida},
  booktitle={Zoological science},
Abstract A 224 bp fragment of the mitochondrial cytochrome b gene has been amplified from a 30-year-old mummy-like specimen of the Japanese river otter Lutra nippon by polymerase chain reaction (PCR). The amplified products were subcloned in the Smal site of pUC18 and sequenced. The sequence was different from those of the congeneric Eurasian otters Lutra lutra (Latvia) and Lutra lutra (China) in 7-9 nucleotides, all of which were located at the third position of a codon and identified as… 

What is the taxonomic status of East Asian otter species based on molecular evidence?: focus on the position of the Japanese otter holotype specimen from museum

The phylogenetic relationship among the genus Lutra and taxonomic status of L. nippon was assessed by using the complete sequences of cytochrome b gene of its holotype, and it was suggested that designation of Japanese otter as a separate species from L. lutra will be reconsidered.

Molecular DNA systematic analyses of East Asian mammals: Sequence Variation of cytochrome b Gene and Control Region of Mitochondrial DNA of Common Otter, Lutra lutra lutra L. (Mammalia, Carnivora) from Korea

Although further molecular and morphological studies with specimens from eastern Asia including Amur region and northeast China are needed, it is possible that the Korean common otter might be closer or identical to the far‐eastern Asian common Otter, L. amurensis Dybowski.

Evaluating the Phylogenetic Status of the Extinct Japanese Otter on the Basis of Mitochondrial Genome Analysis

The Japanese otter lived throughout four main Japanese islands, but it has not been observed in the wild since 1979 and was declared extinct in 2012, and the complete mitochondrial genome of two Japanese otters caught in Kanagawa and Kochi prefectures and five Eurasian otters is determined.

Identification of Two Tentative Strains of European Subspecies of the Eurasian River Otter Lutra lutra lutra (Linnaeus, 1758) from the Partial Mitochondrial Cytochrome b Gene

Results suggest that there are slight differences between the Aand B-line individuals used in the present study, however, further investigations, including re-evaluation of the taxonomy of L. lutra lutra subspecies, are needed to clarify the differences.

Evolutionary History of the Extinct Otter Lived in Japanese Islands

The mitochondrial genome sequence of the two Japanese otter museum specimens was determined, using next-generation sequencing technology, and the phylogenetic status of these specimens in the clade of Lutrinae was evaluated, suggesting that the Japanese Otter is of the genus Lutra and that two genetically divergent lineages exist among the Japanese otters living on Honshu and Shikoku.

Craniometrical variability of the Eurasian otter (Lutra lutra: Carnivora: Mustelidae) from the Northern Eurasia

The analysis of morphometric variability of the Eurasian otter (Lutra lutra) was carried out based on 350 skulls from geographically distant localities throughout the distribution range of this

Geographic variation of craniodental morphology of the Eurasian otter (Lutra lutra) in East Asia

Comparisons of the Eurasian otter populations revealed significant differences in their craniodental morphology, mostly at the snout and parietal regions.

Late pleistocene Ursidae and Mustelidae remains (Mammalia, Carnivora) from Geographical Society Cave in the Russian Far East

  • G. Baryshnikov
  • Environmental Science
    Proceedings of the Zoological Institute RAS
  • 2015
The paleontological collection from Geographical Society Cave located in southern part of Primorskii Territory is found to comprise 5 species of ursids and mustelids: Ursus arctos, Meles anakuma,

A new timeframe for the diversification of Japan’s mammals

A molecular clock is applied to the genetic divergences between Japanese mammalian taxa and their sister mainland taxa to test the late Pleistocene land-bridge colonization hypothesis, implicating a colonization timeframe that is older than posited by the current paradigm based on fossil evidence.

Lutra lutra (Carnivora: Mustelidae)

Lutra lutra, commonly known as the Eurasian otter, is the most widely distributed of the lutrinids (otters) and is primarily a piscivorous predator but also preys on amphibians, crustaceans, small mammals, birds, and reptiles.



A molecular phylogeny of the family Mustelidae (Mammalia, Carnivora), based on comparison of mitochondrial cytochrome b nucleotide sequences.

The molecular phylogenetic tree indicated a clear separation of five genera: Mustela and Martes from the subfamily Mustelinae, Lutra and Enhydra from thesubfamily Lutrinae, and Meles fromThe subfamily Melinae.

Mitochondrial DNA-like sequence in the nuclear genome of an akodontine rodent.

When probed with both functional and nonfunctional gene fragments, MboI restriction digests revealed the same pattern, providing further evidence that the nonfunctional copy must be located in the nucleus.

Dynamics of mitochondrial DNA evolution in animals: amplification and sequencing with conserved primers.

The polymerase chain reaction is used to amplify homologous segments of mtDNA from more than 100 animal species, including mammals, birds, amphibians, fishes, and some invertebrates, and the unexpectedly wide taxonomic utility of these primers offers opportunities for phylogenetic and population research.

Mammal Species of the World: A Taxonomic and Geographic Reference

The third edition of Wilson and Reeder's Mammal Species of the World includes detailed information on nomenclature and, for the first time, common names.

A myoglobin evolved from indoleamine 2,3-dioxygenase.

Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified.

A report on the river otter in Asahikawa (in Japanese). Department of Health and Environment, Hokkaido Government

  • 1990

PHYLIP (Phylogeny Inference Package) version

  • 1993