Phylogenetic Diversity, Host-Specificity and Community Profiling of Sponge-Associated Bacteria in the Northern Gulf of Mexico

@inproceedings{Erwin2011PhylogeneticDH,
  title={Phylogenetic Diversity, Host-Specificity and Community Profiling of Sponge-Associated Bacteria in the Northern Gulf of Mexico},
  author={Patrick M Erwin and Julie B. Olson and Robert W. Thacker},
  booktitle={PloS one},
  year={2011}
}
BACKGROUND Marine sponges can associate with abundant and diverse consortia of microbial symbionts. However, associated bacteria remain unexamined for the majority of host sponges and few studies use phylogenetic metrics to quantify symbiont community diversity. DNA fingerprinting techniques, such as terminal restriction fragment length polymorphisms (T-RFLP), might provide rapid profiling of these communities, but have not been explicitly compared to traditional methods. METHODOLOGY… CONTINUE READING

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DNA fingerprinting techniques , such as terminal restriction fragment length polymorphisms ( T - RFLP ) , might provide rapid profiling of these communities , but have not been explicitly compared to traditional methods .
DNA fingerprinting techniques , such as terminal restriction fragment length polymorphisms ( T - RFLP ) , might provide rapid profiling of these communities , but have not been explicitly compared to traditional methods .
We investigated the bacterial communities associated with the marine sponges Hymeniacidon heliophila and Haliclona tubifera , a sympatric tunicate , Didemnum sp . , and ambient seawater from the northern Gulf of Mexico by combining replicated clone libraries with T - RFLP analyses of 16S rRNA gene sequences .
We investigated the bacterial communities associated with the marine sponges Hymeniacidon heliophila and Haliclona tubifera , a sympatric tunicate , Didemnum sp . , and ambient seawater from the northern Gulf of Mexico by combining replicated clone libraries with T - RFLP analyses of 16S rRNA gene sequences .
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