Phylogenetic Distribution of Unusual Triheme to Tetraheme Cytochrome Subunit in the Reaction Center Complex of Purple Photosynthetic Bacteria

  title={Phylogenetic Distribution of Unusual Triheme to Tetraheme Cytochrome Subunit in the Reaction Center Complex of Purple Photosynthetic Bacteria},
  author={Yusuke Tsukatani and Katsumi Matsuura and Shinji Masuda and Keizo Shimada and Akira Hiraishi and Kenji V P Nagashima},
  journal={Photosynthesis Research},
To understand the evolutionary relationship between triheme and tetraheme cytochrome subunits in the reaction center complex, genes located downstream of that coding for the M subunit of the reaction center complex (pufM) were amplified by PCR and analyzed in six established and two unidentified species of the genus Rhodovulum and five species of the genus Rhodobacter. All the Rhodovulum species tested had the pufC gene coding for the reaction-center-bound cytochrome subunit, while all the… 

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The nucleotide sequence of the pufoperon, which contains the genes encoding the B870 light-harvesting protein and the reaction center complex of the purple photosynthetic bacterium, Rhodovulum sulfidophilum, was determined and it was revealed that this cytochrome contains only three possible heme-binding motifs; the tetraheme cy tochrome subunits was not present.

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Phylogenetic analysis of the light-harvesting proteins indicated that the LH1 α and β subunits of Rsp.

Mutational analyses of the photosynthetic reaction center-bound triheme cytochrome subunit and cytochrome c2 in the purple bacterium Rhodovulum sulfidophilum.

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Evolutionary Relationships between Reaction Center Complexes with and without Cytochrome c Subunits in Purple Bacteria

The major difference between the two types of RC is only in the presence or absence of the cytochrome subunit and the structure of the other three peptides with pigments and quinones is similar to each other.

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The amino acid sequences of the reaction center-bound cytochrome subunit of six species of purple bacteria were compared. Amino acid residues thought to be important in controlling the redox midpoint

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