Phylogenetic Analysis of Methanogens in the Pig Feces

@article{Mao2011PhylogeneticAO,
  title={Phylogenetic Analysis of Methanogens in the Pig Feces},
  author={Sheng-yong Mao and Cui-Feng Yang and Wei-yun Zhu},
  journal={Current Microbiology},
  year={2011},
  volume={62},
  pages={1386-1389}
}
In order to assess methanogen diversity in feces of pigs, archaeal 16S rRNA gene clone libraries were constructed from feces of the pig. After the amplification by PCR using primers Met86F and Met1340R, equal quantities of PCR products from each of the five pigs were mixed together and used to construct the library. Sequence analysis showed that the 74 clones were divided into ten phylotypes as defined by RFLP analysis. Phylogenetic analysis showed that three phylotypes were most closely… CONTINUE READING

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Isolation and characterization of methanogens from animal feces

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  • Syst Appl Microbiol
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