Phycobilisomes of Porphyridium cruentum. I. Isolation.

@article{Gantt1972PhycobilisomesOP,
  title={Phycobilisomes of Porphyridium cruentum. I. Isolation.},
  author={Elisabeth Gantt and Claudia A. Lipschultz},
  journal={The Journal of cell biology},
  year={1972},
  volume={54 2},
  pages={
          313-24
        }
}
A procedure was developed for the isolation of phycobilisomes from Porphyridium cruentum. The cell homogenate, suspended in phosphate buffer (pH 6.8), was treated with 1% Triton X-100, and its supernatant fraction was centrifuged on a sucrose step gradient. Phycobilisomes were recovered in the 1 M sucrose band. The phycobilisome fraction was identified by the characteristic appearance of the phycobilisomes, and the absorbance of the component pigments: phycoerythrin, R-phycocyanin, and… CONTINUE READING

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