Photosynthetic nitrate assimilation in cyanobacteria

  title={Photosynthetic nitrate assimilation in cyanobacteria},
  author={Enrique Flores and José E. Frías and Luis M. Rubio and Antonia Herrero},
  journal={Photosynthesis Research},
Nitrate uptake and reduction to nitrite and ammonium are driven in cyanobacteria by photosynthetically generated assimilatory power, i.e., ATP and reduced ferredoxin. High-affinity nitrate and nitrite uptake takes place in different cyanobacteria through either an ABC-type transporter or a permease from the major facilitator superfamily (MFS). Nitrate reductase and nitrite reductase are ferredoxin-dependent metalloenzymes that carry as prosthetic groups a [4Fe–4S] center and Mo-bis… 

Induction of the nitrate assimilation nirA operon and protein-protein 1 interactions in the maturation of nitrate and nitrite reductases in the 2 cyanobacterium Anabaena

Bacterial two-hybrid analysis confirmed possible NirA-NirB and NarB- NarM interactions suggesting that development of both nitrite reductase and nitrate reduct enzyme activities in cyanobacteria involves physical interaction of the corresponding enzymes with their 35 cognate partners, NirB and narM, respectively.

Induction of the Nitrate Assimilation nirA Operon and Protein-Protein Interactions in the Maturation of Nitrate and Nitrite Reductases in the Cyanobacterium Anabaena sp. Strain PCC 7120

Bacterial two-hybrid analysis confirmed possible NirA-NirB and NarB-NarM interactions, suggesting that the development of both nitrite reductase and nitrate reduct enzyme activities in cyanobacteria involves physical interaction of the corresponding enzymes with their cognate partners, NirB andNarM, respectively.

Negative Regulation of Expression of the Nitrate Assimilation nirA Operon in the Heterocyst-Forming Cyanobacterium Anabaena sp. Strain PCC 7120

Because NirB is also needed to attain high levels of nitrite reductase activity, NirA appears to be a negative element in the nitrate regulation of expression of the nirA operon in Anabaena sp.

Bacterial nitrate assimilation: gene distribution and regulation.

Examination of currently available prokaryotic genome sequences suggests that assimilatory nitrate reductase (Nas) systems are widespread phylogenetically in bacterial and archaeal heterotrophs.

Inactivation of nitrate reductase alters metabolic branching of carbohydrate fermentation in the cyanobacterium Synechococcus sp. strain PCC 7002

The absence of nitrate reductase eliminated the inductive effect of nitrates addition on rerouting carbohydrate catabolism from glycolysis to the oxidative pentose phosphate (OPP) pathway, indicating that intracellular redox poise and not nitrate itself acts as the control switch for carbon flux branching between pathways.

A recently evolved diflavin-containing monomeric nitrate reductase is responsible for highly efficient bacterial nitrate assimilation

The characterization of a novel NAS encoded by Mycolicibacterium smegmatis Msmeg_4206, designated NasN, is reported, which differs from the canonical NASs in its structure, electron transfer mechanism, enzymatic properties, and phylogenetic distribution.

Transcription factor NtcB specifically controls the nitrate assimilation genes in the marine cyanobacterium Synechococcus sp. strain PCC 7002

Results demonstrate that NtcB specifically controls the transcription of the three genes for nitrate assimilation in this marine cyanobacterium.

Genetic responses to carbon and nitrogen availability in Anabaena.

The mechanisms of assimilation of ammonium, nitrate, urea and N2 , the latter involving heterocyst differentiation, are summarized and aspects of CO2 assimilation that involves a carbon concentration mechanism are described.

Hfq Is Required for Optimal Nitrate Assimilation in the Cyanobacterium Anabaena sp. Strain PCC 7120

The results indicate that the RNA chaperone Hfq is involved in the regulation of the nir operon, although the mechanism for this regulation is still unknown.

Transcriptional and translational adaptation to aerobic nitrate anabolism in the denitrifier Paracoccus denitrificans

Transcriptional adaptation to nitrate-dependent anabolism by Paracoccus denitrificans PD1222 was studied and the existence of a regulation mechanism for nitrate assimilation in P. denitRificans, by which nitrate induction operates at both transcriptional and translational levels, is proposed.



Nitrate/Nitrite Assimilation System of the Marine Picoplanktonic Cyanobacterium Synechococcus sp. Strain WH 8103: Effect of Nitrogen Source and Availability on Gene Expression

  • C. BirdM. Wyman
  • Environmental Science, Biology
    Applied and Environmental Microbiology
  • 2003
Nitrogen depletion led to the transient up-regulation of ntcA, nirA,nrtP, narB, and glnA in what appears to be an NtcA-dependent manner, highlighting significant differences in nitrogen control in this cyanobacteria.

Regulation of nitrate reductase levels in the cyanobacteria Anacystis nidulans, Anabaena sp. strain 7119, and Nostoc sp. strain 6719

In the unicellular species Anacystis nidulans, nitrate reductase was repressed in the presence of ammonium but de novo enzyme synthesis took place in media containing either nitrate or not nitrogen source, indicating that nitrate was not required as an obligate inducer.

Nitrate assimilation gene cluster from the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120

A region of the genome of the filamentous, nitrogen-fixing, heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120 that contains a cluster of genes involved in nitrate assimilation has been

Monomeric NarB Is a Dual-Affinity Nitrate Reductase, and Its Activity Is Regulated Differently from That of Nitrate Uptake in the Unicellular Diazotrophic Cyanobacterium Synechococcus sp. Strain RF-1

Under both nitrate-induced and uninduced conditions, the in situ NR activity exhibited similar biphasic kinetics for nitrate, and the recombinant NR encoded by the narB gene of Synechococcus sp.

Requirement of the regulatory protein NtcA for the expression of nitrogen assimilation and heterocyst development genes in the cyanobacterium Anabaena sp. PCC7120

Results showed that the ntcA gene product, NTCA, is required in Anabaena sp.

A Novel Nitrate/Nitrite Permease in the Marine CyanobacteriumSynechococcus sp. Strain PCC 7002

ABSTRACT The nrtP and narB genes, encoding nitrate/nitrite permease and nitrate reductase, respectively, were isolated from the marine cyanobacterium Synechococcus sp. strain PCC 7002 and

Ferredoxin-dependent photosynthetic reduction of nitrate and nitrite by particles of anacystis nidulans

In its presence, the alga particles catalyze the gradual photoreduction of nitrate to nitrite and ammonia, a process that can thus be considered as one of the most simple and relevant examples of Photosynthesis.

Tuning a Nitrate Reductase for Function

The data suggests that during the catalytic cycle nitrate will bind to the Mo5+ state of NarB in which the enzyme is minimally two-electron-reduced, consistent with the coupling of narB to low potential ferredoxins in the cell cytoplasm.

Regulation of Nitrite Reductase in the Cyanobacterium Anacystis nidulans

De novo synthesis of nitrite reductase occurred in the absence of an added nitrogen source, although enzyme activity was higher when the medium contained either NO3 − or NO2 −, suggesting a coordinate regulation of synthesis.

NasFED Proteins Mediate Assimilatory Nitrate and Nitrite Transport in Klebsiella oxytoca(pneumoniae) M5al

Growth tests coupled with nitrate and nitrite uptake assays revealed that the nasFED genes are essential for nitrate transport and participate in nitrite transport as well, and Interestingly, the delta nasF strain exhibited leaky phenotypes, suggesting that the NasED proteins are not fully dependent on the NasF protein.