Photosynthesis in Isolated Chloroplasts of the Crassulacean Acid Metabolism Plant Sedum praealtum.

@article{Spalding1980PhotosynthesisII,
  title={Photosynthesis in Isolated Chloroplasts of the Crassulacean Acid Metabolism Plant Sedum praealtum.},
  author={Martin H. Spalding and Gerald E. Edwards},
  journal={Plant physiology},
  year={1980},
  volume={65 6},
  pages={
          1044-8
        }
}
Intact chloroplasts were isolated from protoplasts of the Crassulacean acid metabolism plant Sedum praealtum D.C. Typical rates of CO(2) fixation or CO(2)-dependent O(2) evolution ranged from 20 to 30 micromoles per milligram chlorophyll per hour and could be stimulated 30 to 50% by several Calvin cycle intermediates. The pH optimum for CO(2) fixation was 7.0 to 7.6 with considerable activity as low as pH 6.4. Low concentrations of orthophosphate (Pi) (optimum 0.4 millimolar) stimulated… CONTINUE READING
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