Photosynthesis and apparent affinity for dissolved inorganic carbon by cells and chloroplasts of Chlamydomonas reinhardtii grown at high and low CO2 concentrations

@article{Sltemeyer1988PhotosynthesisAA,
  title={Photosynthesis and apparent affinity for dissolved inorganic carbon by cells and chloroplasts of Chlamydomonas reinhardtii grown at high and low CO2 concentrations},
  author={Dieter F. S{\"u}ltemeyer and Gerd Kl{\"o}ck and Karlheinz Kreuzberg and Heinrich P. Fock},
  journal={Planta},
  year={1988},
  volume={176},
  pages={256-260}
}
Chloroplasts with high rates of photosynthetic O2 evolution (up to 120 μmol O2· (mg Chl)-1·h-1 compared with 130 μmol O2· (mg Chl)-1·h-1 of whole cells) were isolated from Chlamydomonas reinhardtii cells grown in high and low CO2 concentrations using autolysine-digitonin treatment. At 25° C and pH=7.8, no O2 uptake could be observed in the dark by high- and low-CO2 adapted chloroplasts. Light saturation of photosynthetic net oxygen evolution was reached at 800 μmol photons·m-2·s-1 for high- and… CONTINUE READING
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