Photophysics of the red chromophore of HcRed: evidence for cis-trans isomerization and protonation-state changes.

@article{Mudalige2010PhotophysicsOT,
  title={Photophysics of the red chromophore of HcRed: evidence for cis-trans isomerization and protonation-state changes.},
  author={Kumara Mudalige and Satoshi Habuchi and Peter M. Goodwin and Ranjith Krishna Pai and Frans C. De Schryver and Mircea Cotlet},
  journal={The journal of physical chemistry. B},
  year={2010},
  volume={114 13},
  pages={4678-85}
}
HcRed is a dimeric intrinsically fluorescent protein with origins in the sea anemone Heteractis crispa. This protein exhibits deep red absorption and emission properties. Using a combination of ensemble and single molecule methods and by varying environmental parameters such as temperature and pH, we found spectroscopic evidence for the presence of two ground state conformers, trans and cis chromophores that are in thermal equilibrium and that follow different excited-state pathways upon… CONTINUE READING