Photophysical properties of popular fluorescent adenosine nucleotide analogs used in enzyme mechanism probing.

@article{Leskovar2008PhotophysicalPO,
  title={Photophysical properties of popular fluorescent adenosine nucleotide analogs used in enzyme mechanism probing.},
  author={Adriane Leskovar and Jochen Reinstein},
  journal={Archives of biochemistry and biophysics},
  year={2008},
  volume={473 1},
  pages={
          16-24
        }
}
Fluorescent nucleotide analogs are widely used in mechanistic studies of nucleotide binding and utilizing proteins. We describe here an overview of the photophysical parameters of the most popular nucleotide analogs that have a fluorescent N-methylanthraniloyl-group attached at various positions of the nucleotide. Steady state absorption and fluorescence spectra of free chromophores depend on the type of modification (ribose, base or phosphate moiety) and the addition of proteins suggests that… CONTINUE READING
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